Morphological variation of the foraminifer Ammonia beccarii (Linné) from the Atlantic coast of the United States

24 p., 4 fig.http://paleo.ku.edu/contributions.htm

PaleoBank, A Relational Database for Invertebrate Paleontology: The Data Model

7 p., 1 fig., 2 tables.http://paleo.ku.edu/contributions.htm

Visual totality of rewards-based crowdfunding pitch videos: disentangling the impact of peak negative affective visual expression on funding outcomes

In this study, we introduce visual totality of a crowdfunding pitch video which considers not only visual segments with human faces but also segments without human faces. Drawing from Emotions as Social Information (EASI) theory and expression theory, we analyze more than 4 million frames in 3184 Indiegogo rewards-based crowdfunding pitch videos using the ResNet 50 deep neural network. Results indicate that the impact of peak negative affective visual expression on funding performance is stronger than that of its positive counterpart for both segments with and without human faces. Additionally, the influence of peak negative affective visual expression from human faces is stronger in the first half (vs. the second half) of the pitch video. Further, we found a substitute moderating effect between the peak negative affective visual expression from segments with and without human faces on funding performance. We conducted an additional data collection to ascertain that pain points serve as the underlying mechanism through which negative affective visual expressions related to funding outcome. We discuss the theoretical and practical implications of our study to the crowdfunding literature and the broader research on entrepreneurial resource acquisition.Submitted/Accepted versio

Exploring the surface epitope and nuclear localization analysis of porcine circovirus type 3 capsid protein

Abstract Porcine circovirus 3 (PCV3) is a newly emerging virus associated with porcine dermatitis and nephropathy syndrome (PDNS) and reproductive disorders, impacting global pig populations. Porcine circoviruses contain two major open reading frames (ORFs), and the ORF2 encodes the viral capsid protein (Cap). Cap is the most antigenic structural protein and an ideal candidate for the development of vaccines and diagnostic reagents. This study generated a monoclonal antibody (MAb) specific to PCV3 Cap, MAb CCC160, for diagnosis and pathogenesis studies of this novel virus. The MAb specifically recognized PCV3-infected swine lymph node tissue in an immunohistochemical analysis confirming its clinical diagnostic potential. In addition, a novel linear B-cell epitope recognized by MAb CCC160 was identified at the amino acid region 120–134 of Cap. Nuclear localization analysis of PCV3 Cap revealed a potential nuclear localization signal (NLS) in the middle region (aa 131–143) in addition to the dominant N-terminal NLS that is already known. A cell viability assay further demonstrated that the cytotoxicity of PCV3 Cap is correlated with its nuclear localization, indicating a crucial role of Cap in the pathogenic mechanism of PCV3. A full-length construct of PCV3 Cap was successfully expressed using a baculovirus expression system and purified recombinant proteins self-assembled into virus-like particles (VLPs). The protein constitution of the VLPs was confirmed by MAb CCC160 recognition, indicating the correct conformation and specificity of VLP and exhibiting the linear epitope aa 120–134 on the VLP surface. These results provide insights for developing diagnostic tools and potential VLP vaccines for PCV3, revealing its pathogenesis and antigenic properties

Phylogenetic analysis of classical swine fever virus isolated from Taiwan

Abstract By analyzing the E2 sequences of classical swine fever virus from field outbreaks in Taiwan during 1993-2001, three virus populations with distinct genotypes were determined including one historical (subgroup 3.4) and two exotic (subgroup 2.1) strains. The first subgroup 2.1 virus was isolated in 1994 and further sporadic outbreaks occurred after 1996. Phylogenetic analysis using the E2 region has segregated the Taiwanese strains of 2.1virus into two different genotypes (termed 2.1a and 2.1b). The 2.1b viruses were only isolated in 2001 and shared approximately 94.8% nucleotide identities to the 2.1a viruses in the total genomic sequences. The results suggest that the 2.1a and 2.1b viruses may be introduced from different origins.

Performance of preimplantation genetic testing for aneuploidy in IVF cycles for patients with advanced maternal age, repeat implantation failure, and idiopathic recurrent miscarriage

Objective: The primary objective of this study was to investigate whether preimplantation genetic testing for aneuploidy (PGT-A) of blastocysts through array comparative genomic hybridization (aCGH) improves live birth rates (LBR) in IVF cycles for patients with high prevalence of aneuploidy. Materials and Methods: This study included 1389 blastocysts with aCGH results derived from 296 PGT-A cycles in IVF patients with advanced maternal age (AMA) (n = 87, group A), those with repeated implantation failure (RIF) (n = 82, group B), those with recurrent miscarriage (RM) (n = 82, group C), and oocyte donors (OD) (n = 45, young age, as a control group). Another 61 AMA patients without PGT-A procedures were used as a control group for group A. Vitrification was performed after blastocyst biopsy, and thawed euploid embryos were transferred in a nonstimulated cycle. Results: For the AMA group, a significant increase in LBRs was found in the PGT-A group compared with the non–PGT-A group (54.1% vs. 32.8%, p = 0.018). Consistent LBRs (54.1%, 51.6%, 55.9%, and 57.1%, respectively, in group A, B, C, and young age group) were obtained for all the indications. Conclusions: LBRs can be improved using PGT-A of blastocysts with aCGH in IVF cycles for patients with a high rate of aneuploidy, especially for patients with AMA. Keywords: Array comparative genomic hybridization, Blastocyst, Preimplantation genetic testing for aneuploid